Thrombus Formation Processes are dependent on Endothelial Injuries: Examined by In vivo Two-photon Molecular Imaging and Laser Manipulation

Aim: The cellular mechanisms of thrombotic diseases associated with cardiovascular events remains unclear, largely because of an inability to visualize thrombus formation. Thus, we developed in vivo imaging technique based on singleand multi-photon microscopy and laser injuries technique to revealed the multicellular processes during thrombus development. Methods: We visualized the cell dynamics including single platelet behavior, and assessed dynamic cellular interplay in two thrombosis models using two photon microscopy to CAG-eGFP mice in which GFP was expressed ubiquitously. Hoechst was injected to visualize nucleus, and dextran to blood flow. Mice with anestesia is set on to inverted microscopy, and visualization was performed through small incision. (Figure a, b).